Cancer cell invasion takes place at the cancer-host interface and is a prerequisite for distant metastasis. The relationships between current biologic and clinical concepts like cell migration modes, tumor budding and epithelial-mesenchymal transition (EMT) remains unclear in several aspects, especially for the "real" situation in human cancer. We develop a novel method that provides exact three-dimensional information on both microscopic morphology and gene expression over a virtually unlimited spatial range by reconstruction from serial immunostained tissue slices. Quantitative 3D assessment of tumor budding at the cancer-host interface in human pancreatic, colorectal, lung and breast adenocarcinoma suggests collective cell migration as the mechanism of cancer cell invasion, while single cancer cell migration seems to be virtually absent. Budding tumor cells display a shift towards spindle-like as well as rounded morphology. This is associated with decreased E-Cadherin staining intensity and a shift from membranous to cytoplasmic staining as well as increased nuclear ZEB1 expression.